|Statement||editors, P.H. Seidl, K.H. Schleifer.|
|Contributions||Seidl, P. H. 1948-, Schleifer, Karl H., International Workshop on the Biological Properties of Peptidoglycan (2nd : 1985 : Munich, Germany)|
|LC Classifications||QP702.P47 B56 1986|
|The Physical Object|
|Pagination||xiv, 436 p. :|
|Number of Pages||436|
|LC Control Number||86019791|
Preparation of cell wall peptidoglycan. Cell wall peptidoglycan was essentially prepared by the methodofKolenbranderandEnsign (8). (i) Step 1. A 1-g sample of lyophilized cells was suspended in a ml portion of distilled water and mixedwithanequalvolumeof2% cell suspension was gently shaken at 37°C for 18 h. Duringthe treatment Cited by: Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickness, freed from axial filaments and an envelope sheath. An isolated peptidoglycan fraction was mainly composed of glucosamine, muramic acid, alanine, glutamic acid, ornithine, and glycine in molar ratios of Cited by: Biological properties of bacterial peptidoglycan. Johannsen L(1). Author information: (1)Institute for Chemotherapy, Bayer AG, Wuppertal, Germany. Synthetic and natural muramyl peptides have a variety of biological actions in mammals, including the Cited by: The peptidoglycan cell wall surrounds the cytoplasmic membrane and prevents osmotic lysis. Peptidoglycan is composed of interlocking chains of building blocks called peptidoglycan monomers. In order to grow following binary fission, bacteria have to synthesize new peptidoglycan monomers in the cytoplasm.
A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickness, freed from axial filaments Cited by: Purchase Biological Properties - 1st Edition. Print Book & E-Book. ISBN , Book Edition: 1. from book Bioaerosol Detection Technologies of peptidoglycan surrounded by an outer membrane containing lipopolysaccharides 3 Physical and Biological Properties of Author: Jakob Löndahl. Chemical and biological properties of a peptidoglycan isolated from Treponema pallidum Kazan Article (PDF Available) in Infection and Immunity 31(2) March with 50 Reads.
Most bacteria possess an external skeleton composed of peptidoglycan. As the name implies, peptidoglycan consists of glycan chains to which short peptide stems are bound. Peptidoglycan is insoluble in non-hydrolysing solvents and it is referred to Cited by: Biological properties of peptidoglycan: proceedings: second international workshop, Munich, Federal Republic of Germany, May , Author: P H Seidl ; Karl H Schleifer. A water-soluble tuberculin-active peptidoglycan (TAPG) with a molecular weight of ca. 28, to 30, was isolated from the culture filtrate of Mycobacterium tuberculosis. TAPG was approximately four to five times more potent than tuberculin purified protein derivative S in guinea pigs sensitized with M. tuberculosis or M. bovis (freeze-dried BCG).Cited by: 2. Bacterial pathogens rely on a variety of virulence factors to establish the colonization of a new niche. Although peptidoglycan and its muropeptide derivatives have been known to possess potent biological properties, until recently the molecular bases were poorly by: